Part:BBa_K875008:Experience

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Applications of BBa_K875008

Trieste 2012

M15 bacteria cells (containing the pREP4 plasmid coding for the Lac repressor) are transformed with this Biobrick that resides on pSB1C3 plasmid.

After an over night liquid culture (LB broth) the OD600 absorbance is recorded and the culture is diluted to 0,1 and the batch is splitted into 6 flask – 20ml each. Three of these are induced with IPTG 1mM and the others are not induced.

The OD 600 is then recorded each hour.

It is possible to observe that not induced cells growth up to OD 1, instead the induced ones do not growth during the time, demonstrating the inducible promoter and toxin efficiency.

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